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After incubation, cells were pelleted (500 g, 5 min, 4 °C) and the supernatant was filtered through a 5 µm pore sterile filter (Sterile Millex Filter Unit, Millipore)

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For FY 2024, for all IPPS hospitals (including Puerto Rico hospitals) whose wage indexes are less than or equal to 1.0000, we are proposing to apply the wage index to a labor-related share of 62 percent of the national standardized amount