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Moreover, we used the TNFR1 inhibitor R-7050 to verify that although the protein level of TNFR1 was not changed (Fig

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Tumor spheroid viability assay K1 cells were incubated to form spheroids (~ 7 days) in ultra-low binding 96-well plates for viability assay and in ultra-low binding 24-well plate for imaging with PI staining